Figure 3.

TTFields treatment induces gene expression changes and downregulates BRCA1 DNA damage repair pathway. (a) Supervised clustering using a 1083 gene (FDR<0.01) signature that segregates cell lines by response to TTFields exposure. The heatmap scale is based upon log2 values of normalized gene expression. Clustering analysis of differentially expressed genes after TTFields treatment revealed that (b) 628 genes (FDR<0.05) responded to TTFields in the more responsive cell lines and (c) 1039 genes (FDR<0.05) responded to TTFields in the less responsive cell lines. (d) Identification of differentially regulated canonical pathways for TTFields exposure in the more responsive and the less responsive cell lines. Downregulation of the BRCA1 DNA damage repair pathway is more pronounced in the more responsive cell lines compared to the less responsive cell lines, which is denoted by a decrease in the intensity of blue color reflecting the negative z-scores