Figure 5.

TTFields induce DNA damage and slow the repair of IR-induced DSBs. (a) Changes in the mean number of localized 53BP1 and γ-H2AX foci over 48 h. (b) Change in the mean number of γ-H2AX foci over time with TTFields alone (blue lines) and after receiving 2 Gy (red lines), both followed over 48 h. (c) The mean value for residual γ-H2AX foci and localized 53BP1 and γ-H2AX foci at 48 h post-IR for all cell lines. (d) TTFields exposure for 48 h resulted in the induction of chromatid-type aberrations in the panel of NSCLC lines. (e) The frequency of chromosome-type aberrations after a 48 h TTFields exposure in combination with radiation. Error bars represent the S.E.M. of three separate experiments and asterisks represent significant difference (P<0.05) between indicated conditions