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. 2017 Mar 30;8(3):e2720. doi: 10.1038/cddis.2017.137

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Autophagic flux was detected in H1975 cells after rhArg treatment. (a,b) H1975 cells were stained with Cyto-ID and LysoTracker Red after exposed to 2 U/ml of rhArg for the indicated times. Confocal micrographs were taken at × 40. Green (Cyto-ID) and red (LysoTracker Red) dots in cells were counted by the ImageJ software (n=3, means±S.D.). (c,d) H1975 cells were treated with 2 U/ml of rhArg for different time in the presence or absence of 20 μM CQ. Cell lysates were analyzed by immunoblot analysis. Densitometric values were quantified using the ImageJ software and normalized to control. The values of control were set to 1. The data are presented as means±S.D. of three independent experiments