Figure 5.

Suppressing autophagy promoted cytotoxicity and caspase-dependent apoptosis induced by rhArg in NSCLC cells. (a,b) H1975 cells were incubated with 1 U/ml of rhArg for 24 h in the presence or absence of 20 μM CQ or 15 μM LY294002, and the expression of LC3-I/II was measured by western blot analysis. (c) H1975 cells were incubated with 1 U/ml of rhArg for 96 h in the presence or absence of 20 μM CQ. (d) H1975 cells were incubated with 1 U/ml of rhArg for 72 h in the presence or absence of 15 μM LY294002. (e,f) H460 cells were incubated with 2 U/ml of rhArg for 72 h in the presence or absence of 20 μM CQ or 15 μM LY294002. (c–f) The cell viability was determined by MTT assay. Results were expressed as mean±S.D. and analyzed by Student's t-test (two-tailed). *P<0.05, ** P<0.01. (g and h) H1975 cells were incubated with 1 U/ml of rhArg for 24 h in the presence or absence of 20 μM CQ or 15 μM LY294002, and the expression of PARP, cleaved-PARP and cleaved-caspase 3 was measured by western blot analysis