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. 2017 Mar 30;8(3):e2718. doi: 10.1038/cddis.2017.147

Figure 4.

Figure 4

Depletion of Yip1A downregulates the expression of the anti-apoptotic proteins Bcl-2, cIAP2, and Mcl-1, and the phosphorylation of Bcl-2. (a) HeLa cells were transfected with control scramble siRNA or Yip1A siRNA. RT-PCR shows the relative mRNA levels of the indicated anti-apoptotic factors in control and Yip1A-knockdown cells at 48  after siRNA transfection. GAPDH was used for normalization. Data are means±S.D. from three independent experiments; **P<0.01. (b) RT-PCR shows the relative mRNA levels of the indicated proapoptotic factors in control and Yip1A-knockdown HeLa cells at 48 h after siRNA transfection. GAPDH was used for normalization. Data are means±S.D. from three independent experiments. (c) Western blotting shows the relative levels of anti-apoptotic Bcl-2, cIAP2, and Mcl-1 protein in control and Yip1A-knockdown cells at 48 h after siRNA transfection. GAPDH was used for normalization. Data are means±S.D. from three independent experiments; *P<0.05, **P<0.01. (d) Western blotting shows the relative levels of phosphorylated Bcl-2 (pBcl-2) protein in control and Yip1A-knockdown cells at 48 h after siRNA transfection. GAPDH was used for normalization. Data are means±S.D. from three independent experiments; **P<0.01. (e) CaSki cells were transfected with control scramble siRNA or Yip1A siRNA. Western blotting shows the relative levels of anti-apoptotic Bcl-2, pBcl-2, cIAP2, and Mcl-1 protein in control and Yip1A-knockdown cells at 72 h after siRNA transfection. GAPDH was used for normalization. Data are means±S.D. from three independent experiments; **P<0.01