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. 2017 Mar 30;8(3):e2718. doi: 10.1038/cddis.2017.147

Figure 6.

Figure 6

Both the IRE1 and PERK pathways engage in the upregulation of anti-apoptotic proteins and autophagy-related proteins in HeLa and CaSki cells. (a) HeLa cells were transfected with the indicated siRNAs, and cell lysates were prepared at 48 h. Western blotting shows the relative levels of Bcl-2, pBcl-2, cIAP2, and Mcl-1 protein in respective siRNA-transfected cells. GAPDH was used for normalization. Data are means±S.D. from three independent experiments. The significant difference from the control (scramble) was determined using Dunnett's post hoc test; **P<0.01. (b) Western blotting shows relative levels of Atg7, p62, and LC3-II protein, and Atg5–Atg12 conjugate in respective siRNA-transfected HeLa cells. GAPDH was used for normalization. Data are means±S.D. from three independent experiments. The significant difference from the control (scramble) was determined using Dunnett's post hoc test; **P<0.01. (c) Western blotting shows relative levels of cleaved caspase 3 and cleaved PARP protein in respective siRNA-transfected HeLa cells. GAPDH was used for normalization. Data are means±S.D. from three independent experiments. The significant difference from the control (scramble) was determined using Dunnett's post hoc test; **P<0.01. (d) CaSki cells were transfected with the indicated siRNAs, and the cell lysates were prepared at 72 h. Western blotting shows the relative levels of Bcl-2, pBcl-2, cIAP2, and Mcl-1 protein in respective siRNA-transfected cells. GAPDH was used for normalization. Data are means±S.D. from three independent experiments. The significant difference from the control (scramble) was determined using Dunnett's post hoc test; **P<0.01. (e) Western blotting shows relative levels of Atg7, p62, and LC3-II protein, and Atg5–Atg12 conjugate in respective siRNA-transfected CaSki cells. GAPDH was used for normalization. Data are means±S.D. from three independent experiments. The significant difference from the control (scramble) was determined using Dunnett's post hoc test; *P<0.05, **P<0.01. (f) Western blotting shows relative levels of cleaved caspase 3 and cleaved PARP protein in respective siRNA-transfected CaSki cells. GAPDH was used for normalization. Data are means±S.D. from three independent experiments. The significant difference from the control (scramble) was determined using Dunnett's post hoc test; **P<0.01