Figure 8.

Madecassic acid recovered T helper type 17 (Th17)/regulatory T (Treg) balance in DSS-induced mice colitis through the PPARγ/AMPK/ACC1 pathway. Mice were fed 2.5% DSS for 7 days and then normal water for 3 days. Madecassic acid (25 mg/kg) and rosiglitazone (25 mg/kg) were orally administered for 10 days. GW9662 (1 mg/kg) was administered by an intraperitoneal injection. (a) The expression of CD36 and LPL was analyzed by western blot. (b) The expression of p-AMPK, ACC1 and Foxp3 was detected by western blot. (c) The percentages of Th17 and Treg cells were measured by flow cytometry. (d) The levels of IL-17A, IL-17F, IL-21, IL-22 and IL-10 were assessed by real-time PCR. (e) DAI scores. (f) Colon length. (g) MPO activity. (h) H&E scores. GAPDH was used as a cytoplasm marker. The data were expressed as means±S.E.M., n=5–8. ^#P<0.05, ^##P<0.01 versus normal group; *P<0.05, **P<0.01 versus DSS group ^$P<0.05, ^$$P<0.01 versus madecassic acid group