Figure 4.

Depletion of PrP^C increases F-actin formation. (a and b) Immunocytochemical staining for F-actin in ZW and Zpl cells (a), and ZW cells transfected with either scrambled RNA (SCR) or mPrP-targeted siRNA (Si-PrP) (b) using Alexa Fluor 488-phalloidin (green). DAPI (blue) was used to counterstain the nuclei. All pictures are representative of multiple images from three independent experiments (scale bars, 20 μm). The expression of PrP^C was determined by western blot with anti-PrP (3F10) antibody and HSP90 was used as a loading control. (c) The expression of F-actin assessed by a sedimentation assay in ZW, Zpl, and Zpl expressing mPrP cells was analyzed by western blot with anti-β-actin, anti-PrP (3F10) and anti-HSP90 antibodies. The intensities of the bands in each panel were measured and quantified for each group, and the values are expressed as the mean±S.E. of three independent experiments (*P<0.05, n=3)