Figure 6.
PrPC binds to GTP-bound RhoA and p190RhoGAP. (a) ZW cell lysates were preloaded with GDP or GTPγS followed by immunoprecipitation with the anti-RhoA antibody and analyzed by western blot using the anti-PrP (3F10) and anti-RhoA antibodies. WCL, whole-cell lysates. (b) Zpl cell lysates preloaded with GDP or GTPγS were immunoprecipitated with anti-RhoA antibody, incubated with 2 μg of human recombinant PrP (Hu-PrP), and analyzed by western blot with the anti-PrP (3F4) and anti-RhoA antibodies. (c) The co-immunoprecipitation of RhoA or p190RhoGAP using ZW cells transiently transfected with either SCR or Si-PrP was detected by western blot using anti-p190RhoGAP and anti-RhoA antibodies, respectively. The data are expressed as the mean±S.E. of three independent experiments (*P<0.05, n=3)