Figure 7.

The disease-associated mutations of PrP^C impair neurite outgrowth. (a and b) PC12 cells stably expressing either vector, WT, P102L, or MΔ8 were treated with 50 ng/ml NGF for 72 h. (c and d) The cells expressing either vector, WT, P102L, or MΔ8 were incubated with or without 10 μM Y27632 in the presence of NGF. Changes in the cell morphology, neurite length, and neurite numbers were determined under a microscope. The data are expressed as the mean±S.E. of three independent experiments (*P<0.05, **P<0.01, ***P<0.001, n=3)