Figure 1.

High glucose induces miR-27a expression in cultured podocytes. (a) qRT-PCR analysis shows the level of miR-27a in various conditions as indicated. (b) Representative western blotting shows the expression of PPARγ and β-catenin target genes in various conditions as indicated. Cell lysates were immunoblotted with specific antibodies against PPARγ, active β-catenin, snail1, α-SMA, podocin and β-actin. qRT-PCR shows that miR-27a was increased in a (c) time and (d) dose-dependent manner. (e-f) qRT-PCR and (g-h) western blot analyses show the expression level of PPARγ and β-catenin target genes in a time- and dose-dependent manner. (i) PPARγ gene transcription was amplified by miR-27ai and diminished by miR-27am. Mouse podocytes were co-transfected with miR-27ai, miR-27am, and control or wild-type or mutant 3′-UTR of PPARγ and transfection efficiency was evaluated by luciferase reporter assay. *P<0.05; ^#P<0.001. Active β-cat, active β-catenin; CTNNB1, catenin beta-1; HG, high glucose; miR-iNC: miRNA inhibitor negative control; mt: mutant type; NG, normal glucose; wt: wild type