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. 2017 Mar 9;8(3):e2663. doi: 10.1038/cddis.2017.75

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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miR-377 promoted senescence by suppressing DNMT1 expression in HSFs. (a) Cellular senescence was detected by evaluating SA-β-gal-positive cells in young HSFs (PD<10) treated with miR-377 mimics together with control cDNA or DNMT1 cDNA as indicated (left). The positive cell quantification was shown (right; data represent the mean±S.E.M. n=3, *P<0.05). (b) DNMT1, p16, and Rb expressions and phosphorylation level of Rb were detected by western blot in young HSFs (PD<10) treated with miR-377 mimics together with control cDNA or DNMT1 cDNA as indicated (*P<0.05). Representative data was shown. (c) Absorbance at 490 nm was detected by MTS assays in young HSFs (PD<10) treated with miR-377 mimics together with control cDNA or DNMT1 cDNA as indicated (data represented as the mean±S.E.M. n=3 at each time point, *P<0.05). (d) Cellular senescence was detected by evaluating SA-β-gal-positive cells in passage-aged HSFs (PD>50) treated with miR-377 inhibitors together with control shRNA or DNMT1 shRNA as indicated (left). The SA-β-gal-positive ratio was shown (right; data represented as the mean±S.E.M. n=3, *P<0.05). (e) DNMT1, p16, and Rb expressions and phosphorylation level of Rb were detected by western blot in passage-aged HSFs (PD>50) treated with miR-377 inhibitors together with control cDNA or DNMT1 cDNA as indicated (*P<0.05). Representative data was shown. (f) Absorbance at 490 nm was detected by MTS assays in passage-aged HSFs (PD>50) treated with miR-377 inhibitors together with control cDNA or DNMT1 cDNA as indicated (data represented as the mean±S.E.M. n=3 at each time point, *P<0.05)