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. 2017 Mar 9;8(3):e2662. doi: 10.1038/cddis.2017.82

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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E2-ERs promote Nppc/Npr2 levels and maintain NPPC-mediated oocyte meiotic arrest. (a and b) Effects of E2 and ICI182780 on Nppc and Npr2 mRNA levels in follicles. Follicles were cultured in medium containing 0.0 μM (control)–10.0 μM E2 or plus 10 μM ICI182780 (ICI: a nonselective ERα and ERβ antagonist) for 4 h. n=3. (c) Effects of E2 and ICI on Npr2 mRNA levels in COCs. COCs were cultured for 24 h in medium without (control) or with 0.1 μM E2 or plus 0.1–1.0 μM ICI. n=3. (d) Effects of E2 and ICI on NPPC-mediated oocyte meiotic arrest in COCs. COCs were cultured for 24 h and the percentages of oocytes that underwent GVB were determined. E2, 0.1 μM; NPPC, 0.03 μM; ICI, 0.1–1.0 μM. n=4. Data represent the mean±S.E.M. Different letters (a-c) indicate significant differences between groups (P<0.05, ANOVA and Holm–Sidaik test)