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. 2017 Mar 9;8(3):e2662. doi: 10.1038/cddis.2017.82

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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ERKO mouse ovaries show decreased NPPC/NPR2 levels and percentages of meiotic arrested oocytes. (a and b) Expression of Nppc levels in MGCs and Npr2 levels in COCs isolated from 22- to 24-day-old WT and ERKO mouse ovaries. Ovaries were stimulated with PMSG for 46 to 48 h. n=3. (c) A prophase-arrested oocyte (GV) within a large antral follicle of a WT ovary and an oocyte with metaphase I (MI) chromosomes within a large antral follicle of a αERKO ovary. Scale bars: 100 μm. (d) Percentages of oocytes that had resumed meiosis, counted in serial sections of ovaries from WT and αERKO mice. n=9. (e and f) Effects of E2 on Nppc levels in MGCs and Npr2 levels in COCs isolated from WT and ERKO mice. MGCs and COCs were cultured for 24 h in medium without (control) or with 0.1 μM E2. n=3. (g) Effect of E2 on NPPC-maintained oocyte meiotic arrest within COCs isolated from WT and ERKO mice. COCs were incubated in medium containing 0.03 μM NPPC or plus 0.1 μM E2 for 24 h. n=4. Data represent the mean±S.E.M. *P<0.05, **P<0.01 and ***P<0.001 (t-test)