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. 2017 Mar 23;8(3):e2695. doi: 10.1038/cddis.2017.86

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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MDSCs recruit to injured kidney following rapamycin treatment. CD11b⁺ cells were first gated from FSC/SSC, and then Ly-6G⁺Ly-6C^low and Ly-6G⁻Ly-6C^high cell populations were detected within CD11b⁺ cells. (a) The frequency of CD11b⁺Ly-6G⁺Ly-6C^low G-MDSCs, CD11b⁺Ly-6G⁻Ly-6C^high M-MDSCs and total MDSCs in spleen on POD 1 and POD 2 were examined by flow cytometry. (b) The frequency of CD11b⁺Ly-6G⁺Ly-6C^low G-MDSCs, CD11b⁺Ly-6G⁻Ly-6C^high M-MDSCs and total MDSCs in the kidney on POD 1 and POD 2 were examined by flow cytometry. (data were shown as mean±S.D.; n=5 mice per group per time point; POD, postoperative day)