Figure 5.

mTOR signal regulates the induction of MDSCs from bone marrow cells. (a) CD11b⁺ cells were gated first, and Ly-6G⁺Ly-6C^low and Ly-6G⁻Ly-6C^high cell populations were detected within CD11b⁺ cells. In comparison with control, rapamycin treatment directed MDSC induction towards Ly-6G⁺Ly-6C^low G-MDSC subtype. (b) After 100 nM rapamycin or equal volume of DMSO treatment for 4 h, the frequencies of CD4⁺IFN-γ⁺ Th1 cells, CD4⁺IL-17a⁺ Th17 cells and CD8⁺IFN-γ⁺ T cells were examined by using flow cytometry, respectively. (data were shown as mean±S.D.; n=5 duplicate tests)