Figure 2.

Knockdown of NCAPH inhibits colonic cancer cell proliferation. (a) Relative NCAPH mRNA expression in different colonic cell lines: normal human colonic epithelial cell (HCoEpiC) and colorectal cancerous cell lines (LoVo, SW480, SW620 and HCT116). HCoEpiC was set as a control. (b) Relative NCAPH protein expression in different colonic cell lines. Indicated cell lysates were probed with NCAPH and GAPDH antibodies, representative images were shown. (c–-d) Individual NCAPH-shRNA knockdown efficiency was verified by real-time PCR (c) and western blot with NCAPH and GAPDH antibodies (d); scramble shRNA was set as a control. α, anti; Ctr, scramble shRNA control; sh#1, shRNA#1; sh#2, shRNA#2. (e and f) Knockdown of NCAPH significantly inhibited HCT116 (e) and SW480 (f) cell growth. ***P<0.001, t-test