(A) Comparison of the mean firing rate as a function of time of the FSI population on day 1 and day 4 of training. Data are aligned to the first lick during hit trials without laser.
(B) The median level of preparatory FSI activity per animal did not significantly change from day 1 to day 4 (Mann-Whitney test, p = 0.38). The firing rate change is with respect to baseline activity.
(C) The fraction of FSIs per animal that were significantly excited during the preparatory period did not significantly change from day 1 to day 4 (Mann-Whitney test, p = 0.25).
(D) The fraction of FSIs per animal that were significantly inhibited during the preparatory period did not significantly change from day 1 to day 4 (Mann-Whitney test, p = 0.25). Data in (A–D) are derived from non-laser trials, and each data point in (B–D) represents one animal (n = 7 mice per group).
(E) Illustrative model of how the contribution of PV interneurons on MSN activity diminishes relative to other influences (here depicted as external excitatory input) in an experience-dependent manner. Left and right panels represent day 1 and day 4, respectively. Arrow thickness represents the relative strength of the designated pathway.