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. 2016 May 17;8(12):18712–18725. doi: 10.18632/oncotarget.9409

Figure 4. Effect of SERPINA3 Silencing on Melanoma Cell Proliferation.

Figure 4

Panel A. Knockdown of SERPINA3 expression in MMRU cells using synthetic small inhibitor RNA (SiRNA). Realtime PCR verified that SERPINA3 expression was significantly decreased in cultures cells transfected with two individually targeted SiRNA (SiRNA1 and SiRNA2) compared to random scrambled negative control SiRNA. Data represented as mean expression as compared to control +/− standard deviation. A Western blot is also shown from 72hrs after siRNA knockdown. Actin is used as the internal control, and SERPINA3 band seen at ~75kDa is shown. C: control SiRNA, 1: SiRNA1, 2: SiRNA2. Panel B. A Western blot is also shown from 72hrs after siRNA knockdown. Actin is used as the internal control, and SERPINA3 band seen at ~75kDa is shown. C: control SiRNA, 1: SiRNA1, 2: SiRNA2. Panel C. Effect of SERPINA3 silencing on melanoma cell proliferation, measured by MTS assay, represented as mean absorbance +/− standard deviation.