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. 2016 Nov 4;8(12):18773–18791. doi: 10.18632/oncotarget.13095

Figure 3.

Figure 3

(A–C) Classification of effect strength and cell specific pro-apoptotic effects of mmu-miR-96-5p, -1912-5p, -147b-5p and -3073a-3p in human cancer cell lines. Quartile classification of the pro-apoptotic effect intensity regarding the specific apoptosis rate of all 188 miRNA mimics selected from the initial screening in CHO cells (conditions of the experiment described in Figure 1). All 188 miRNA were individually transfected in SGBS, SKOV3, T98G and HCT 116 cells. 25% of the values representing the highest relative effects were classified as “strong”. Values with descending effects were classified in “medium”, “weak” or “no” effect, respectively (A). Venn diagram illustrates overlapping as well as cell line specific pro-apoptotic effects of the 47 miRNAs with the strongest pro-apoptotic properties (B). Pro-apoptotic miRNAs marked in red were analyzed in further detail (C). To compare the effects of the transfected miRNAs a non-targeting control (NT) was used. Statistical analysis was performed by two-way ANOVA followed by Bonferroni post-test [n = 3; Mean ± SD, * p < 0.05; ** p < 0.01; *** p < 0.001; ****p < 0.0001]. #passenger strand (miRBase Version 21).