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. 2017 Jan 27;8(12):18979–18990. doi: 10.18632/oncotarget.14866

Figure 5. Adamts18 deficient mice are highly susceptible to DSS-induced colitis.

Figure 5

DSS colitis model was established in wild-type (WT) mice and Adamts18 knock-out (KO) littermates by administration of 3% DSS solution in drinking water for 7 days. (A) The body weights of the WT and Adamts18 KO mice were monitored during the study period. Weights are expressed as means of individual data relative to initial body weight of each animal. The Adamts18 KO mice suffered from significant body weight loss from day 6. (B) Quantification of colon length of WT and Adamts18 KO mice at time of sacrifice. (C–F) Sera lipopolysaccharides (LPS) and inflammatory cytokine assay. Concentrations of LPS (C), tumor necrosis factor-alpha (TNF-α) (D), interleukin (IL)-11 (E), and IL-4 (F) determined by sandwich ELISA. Each symbol represents one individual. Horizontal bars represent mean value. (G–H) Western blot analysis of the expression level of TNF-α and IL-4 in mice colitis tissues. Columns mean; bars, SD (*P < 0.05, **P < 0.01). These experiments are repeated independently at three times.