Figure 1. CUX1 Knockdown Sensitizes Tumor Cells to Radiation.
Lentivirus expressing a doxycycline inducible shRNA against CUX1 was introduced in tumor cell lines of various tissue of origin to obtain large populations of cells stably carrying the lentiviral vector. Cells were treated with doxycycline (+) or not (−) for 4 days. (A) Total protein extracts were used in immunoblotting analysis using the indicated antibodies. (B) Cells were treated with radiation and then submitted to a clonogenic assay. Cloning efficiency of untreated control cells was set at 100%. Results of triplicate experiments are shown. Error bars represent standard error. ***p < 0.001; **p < 0.01.; *p < 0.05; Student's t-test. (C) Results from clonogenic efficiency data in Figure 1B are represented as line graphs with all un-irradiated cells (−Dox, +Dox) set at 100%. Results of triplicate experiments are shown. Error bars represent standard error. ***p < 0.001; **p < 0.01.; *p < 0.05; Student's t-test. (D) Levels of reactive oxygen species (ROS) were measured by staining with CM-H2DCFDA.