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. 2017 Jan 28;8(12):19021–19038. doi: 10.18632/oncotarget.14875

Figure 6. Cut domain is Necessary to Increase Resistance to Radiation and Repair of Oxidative DNA Damage.

Figure 6

(A) Map showing the recombinant CUX1 proteins C1C2, C1 and C1-2Ala, which contains two point mutations replacing glutamic acid to alanine. The region recognized by the CUX1-861 antibody is shown below the C1C2 map. A coomassie blue stain of the purified proteins is shown. (B) 8-oxoG cleavage assay was performed using 50 nM OGG1 and 100 nM of BSA or C1C2, C1, or C1-2Ala. (C) DLD-1 cells were infected with lentiviruses expressing either nothing (Vector), C1C2-NLS, C1-NLS, or C1-2Ala-NLS. Nuclear extracts were analyzed by immunoblotting using the indicated antibodies. (D) Cells were submitted to ionizing radiation and their clonogenic efficiency was determined. Cloning efficiency of unexposed vector cells was set at 100%. Results of triplicate experiments are shown. Error bars represent standard error. ***p < 0.001; **p < 0.01.; *p < 0.05; Student's t-test. (E) Results from clonogenic efficiency data in Figure 6D are represented as line graphs, with all non-irradiated cells set at 100%. Results of triplicate experiments are shown. Error bars represent standard error. *p < 0.05; Student's t-test comparing C1C2-NLS (1), C1-NLS (2), or C1-2Ala-NLS against the vector.