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. 2017 Feb 7;8(12):19192–19204. doi: 10.18632/oncotarget.15163

Figure 3. OCT4A overexpression significantly enhances tumorigenicity of medulloblastoma cells.

Figure 3

(A) Kinetics of tumor growth after subcutaneous inoculation of medulloblastoma cells in BALB/c nude mice. OCT4A-overexpressing cells generated more developed tumors at a faster pace than corresponding control cells. *p < 0.05, **p < 0.01. (B) Representative images of subcutaneous tumors resected after 90 or 120 days post-injection of Daoy or USP-13-Med cells, respectively. Control USP-13-Med cells and D283Med cells did not generate palpable subcutaneous tumors under the experimental conditions tested. (C) Histological analysis showing typical aggressive features, including extensive necrotic and hemorrhagic areas, in subcutaneous tumors from OCT4A-overexpressing cells. (D) Representative images of histological brain sections from BALB/c nude mice bearing orthotopically implanted medulloblastoma cells. Local tumor cell invasion was typically observed in brain tumors generated from OCT4A-overexpressing cells. (E) Bioluminescence-based detection of medulloblastoma cells orthotopically injected in BALB/c nude mice. Images were taken three or four weeks after the intracerebroventricular injection of Daoy or USP-13-Med cells, respectively. Multiple small tumor foci, including spreading to the spinal cord were more frequent in mice injected with OCT4A-overexpressing cells. (F) Kaplan-Meier curves showing shorter overall survival rates for BALB/c nude mice bearing orthotopically implanted OCT4A-overexpressing cells.