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. 2017 Feb 7;8(12):19218–19235. doi: 10.18632/oncotarget.15168

Figure 1. Upregulation of FAM84B in prostate cancer stem-like cells (PCSLCs).

Figure 1

(A) Affymetrix profiling gene expression in DU145 monolayer and sphere cells. The profiling was carried out three times with duplicates for each repeat. FAM84B mRNA is expressed as fold change compared to monolayer cells. Mean±SD (standard deviation) are graphed. Statistical analysis was performed using 2-tailed Student's t-test. (B, D) Real-time PCR analysis of FAM84B mRNA in DU145 monolayer, DU145 sphere cells (PCSLCs), and the indicated PC lines. β-actin was used as an internal control. Experiments were repeated three times. FAM84B mRNA abundance is graphed as a fold change to DU145 monolayer cells; mean ± SD are graphed. *:p < 0.05 by a 2-tailed Student's t-test. (C) Western blot examination of FAM84B protein expression in DU145 monolayer and sphere, PC3, and LNCaP cells. Experiments were repeated three times; typical images from a single repeat are shown (inset). FAM84B protein levels were normalized to actin; mean±SD are graphed. *p < 0.05 by a 2-tailed Student's t-test in comparison to DU145 monolayer cells. (E) FAM84B protein expression in xenograft tumors produced by either DU145 monolayer or sphere cells. The overall patterns of FAM84B expression at low magnification are presented in Supplementary Figure 2.