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. 2017 Feb 8;8(12):19285–19297. doi: 10.18632/oncotarget.15195

Figure 4. let-7c is involved in CSE-induced increases of c-Myc expression in HBE cells.

Figure 4

Densities of bands were quantified by Eagle Eye II software. GAPDH levels, measured in parallel, served as controls. (A) Schematic graph of the binding sites of let-7c in the 3′UTR of c-Myc. HBE cells were exposed to CSE (0 or 20 μg/mL) for 24 h after they were transfected with 50 nM let-7c mimic or control mimic for 24 h. HBE cells were co-transfected with pmirGLO-c-Myc-3′UTR -WT or pmirGLO-Ctrl and with 50 nM let-7c mimic or control mimic for 24 h, then exposed to CSE (0 or 20 μg/mL) for 24 h. (B) Luciferase activity was measured at 24 h after transfection. Means of triplicate assays with standard deviations were presented. (C) The levels (means ± SD, n = 3) of let-7c were determined by quantitative RT-PCR. **P < 0.05, different from CSE-treated HBE cells in the absence of the let-7c mimic. (D) Western blots and relative protein levels (means ± SD, n = 3) of c-Myc were determined. **P < 0.05, different from CSE-treated HBE cells in the absence of the let-7c mimic.