Skip to main content
. 2016 Dec 27;8(12):19323–19341. doi: 10.18632/oncotarget.14252

Figure 2. Secreted Hsp90 increases prostasphere formation.

Figure 2

A, B. Representative images of prostaspheres generated at P0 (first generation) and P3 (fourth generation) in ARCaPE-LacZ and ARCaPE-eHsp90 (A) and DU145-LacZ and DU145-eHsp90 (B). Bottom panels depict a graphical representation of the spheres generated from P0-P3 when passaged at 2000 cells per well in a 6 well low-attachment plate. The experimental eHsp90-expressing cells were compared to LacZ controls for each respective passage number. C. Immunoblot analysis depicting Snail and EZH2 expression in shRNA transduced ARCaPE-eHsp90 and DU145-eHsp90 cell models in relation to the shGFP control vector. Tubulin is used as a loading control. D, E. Graphical representation of the spheres generated from P0-P2 in ARCaPE-eHsp90 (D) and P0 in DU145-eHsp90 (E) following suppression of either Snail or EZH2. Successive generations in DU145-eHsp90 did not produce spheres. The shSnail and shEZH2 experimentals were statistically compared against shGFP for each given passage number. All statistics were performed using the Student's t-test. * = p<0.05, ** p<0.01.