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. 2016 Dec 27;8(12):19413–19426. doi: 10.18632/oncotarget.14297

Figure 2. Hyperglycemia increased myocardial ALDH2 O-GlcNAc modification and decreased its activity after myocardial ischemia/reperfusion in rats.

Figure 2

A. Overall protein O-GlcNAcylation in myocardial tissue. Tissue lysates were separated by SDS-PAGE, and western blot was performed using specific anti-O-GlcNAc (CDT110.6) antibody. B. ALDH2 O-GlcNAcylation in myocardial tissue. Tissue lysates were immunoprecipitated with specific anti-ALDH2 antibody and then subjected to western blot using anti-O-GlcNAc (CDT110.6) antibody. C. ALDH2 dehydrogenase activity analysis. ALDH2 activity was determined by measuring the conversion of NAD+ to NADH. In each graph, the mean value of Con was expressed as 1 or 100%. IP, immunoprecipitation; IB, immunoblotting; Sham, sham-operated; NS, normal saline; I/R, myocardial ischemia/reperfusion; AHG, acute hyperglycemia; DM, diabetes. Data are means ± SEM of 4 to 5 samples in each group. *P<0.05 vs Sham group; †P<0.05 vs NS+I/R group.