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. 2017 Jan 3;8(12):19507–19521. doi: 10.18632/oncotarget.14470

Figure 1. MiR-24 expression in BCSCs.

Figure 1

A. miR-24 expression analyzed by qRT-PCR in primary breast cancer cells cultured in suspension (stem cell enriched) or adherent (differentiated) conditions. B. qRT-PCR levels of miR-24 in T47D, MCF-7, MDA-MB-231, and BT-549 breast cancer and in differentiated cells. C. T47D, MCF-7, and BT-549 cells were transfected with miR-24 or a scrambled oligonucleotide, grown as mammospheres, and counted after 6 days. D. Primary cells were transfected with miR-24 or scambled oligonucleotide, grown as mammospheres and counted after 6 days. E-F. Western blot showing that miR-24 overexpression in T47D and MCF-7 cells determines an upregulation of the stem markers Nanog, Oct-3/4, and Vimentin, and a downregulation of E-cadherin. In B, C, D, data are mean values ± SD of three independent experiments. Significance was calculated using Student's t-test.*, p<0.05; **, p<0.01. Western blots are from representative experiments.