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. 2017 Jan 19;8(12):19592–19608. doi: 10.18632/oncotarget.14752

Figure 2. Breast fibroblast-derived exosomes are transferred to T47D cells and miRs -21, -143, and -378e are shuttled by CAF exosomes into breast cancer cells.

Figure 2

T47D cells were cultured in the absence (control) or presence of either NF- (patient #6) or CAF- (patient #3) derived PKH26-labeled exosomes for 24 hours. Exosomes were taken up by T47D cells (confocal microscopy, ×60 original magnification). T47D cells were stained using DAPI (nuclei) and ALEXA488-conjugated anti-tubulin antibody. Scale bar: 10μm a. T47D cells were treated 24 hours in the absence (control) or presence of either NF exosomes (patient #6) or CAF exosomes (patients #3, #7 and #8). Real Time PCR was performed to evaluate miRs -21, -143, and -378e levels. Data obtained from three independent experiments and presented as mean value ± SD. P-value calculated using one-way ANOVA followed by Bonferroni's post hoc testing. * p<0.05; ** p<0.01 (over control). § p<0.05; §§ p<0.01 (over NF ex) b. T47D cells were cultured in the absence (control) or presence of exosomes isolated from cy3-miR-CAF#11 (cy3-miRs -21, -143, -378e) for 24 hours. Cy3-labeled miRs were shuttled from CAF#11 exosomes into T47D cells (confocal microscopy, ×60 original magnification). T47D cells were stained using DAPI (nuclei) and ALEXA488-conjugated anti-CD63 antibody. Scale bar: 10μm c.