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. 2017 Jan 19;8(12):19592–19608. doi: 10.18632/oncotarget.14752

Figure 6. miRs -21, -143, and -378e promote anchorage-independent cell growth and invasive capacity.

Figure 6

T47D cells were transfected with scrambled miRs (scr, control), or miRs -21, -143, and -378e, alone or in combination (final concentration: 100nM). After 24h, cells were harvested and cultured in soft agar. After 3 weeks, the colonies were counted a. Scale bar: 100μm b. Invasion assays were performed in BT549 c, d. and MDA-MB-231 e, f. cells transfected with scrambled or miRs -21, -143, and -378e, alone or in combination (final concentration: 100nM). After 48h, cells were harvested and cultured in transwell plates coated with matrigel. Invasive cells were fixed with Crystal Violet solution. Percentage of invasive cells was evaluated by eluting fixed cells with 1% SDS and reading the absorbance at λ570nm. Data were obtained from three independent experiments and presented as mean value ± SD. P-value calculated using Student's t test. * p<0.05; ** p<0.01; *** p<0.001 (over control).