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. 2017 Feb 1;8(12):19712–19722. doi: 10.18632/oncotarget.14966

Figure 3. Global methylation of Sat2, Alu and LINE-1 in ccRCC and adjacent normal tissues.

Figure 3

A. Bisulfite-Modified DNA Sequencing (BMDS) analysis. B. Electrophoresis of COBRA products (Alu DNA sequence + Mbo1 digestion) in agarose gel. C. Rate of DNA methylation in Alu DNA sequence. D. Electrophoresis of COBRA products (LINE-1 DNA sequence + Taq1 digestion) in agarose gel. E. Rate of DNA methylation in LINE-1 DNA sequence. F. Electrophoresis of COBRA products (LINE-1 DNA sequence + TSP509I digestion) in agarose gel. G. Rate of DNA unmethylation in LINE-1 DNA sequence. (*p<0.05).