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. 2017 Feb 1;8(12):19723–19737. doi: 10.18632/oncotarget.14975

Figure 4. miR-320a suppresses GBM cell proliferation by targeting β-catenin and SND1.

Figure 4

A. Growth curves from U87MG and U251 cells transfected with Scr or miR-320a and cotransfected with miR-320a plus plasmid expressing β-catenin (miR-320a+CTNNB1) or SND1 (miR-320a+SND1) assessed by CCK8 assay. B and C. EdU-positive rate (B) and representative images (C) in the indicated cells assessed by EdU assay. D and E. Representative images (left) and percentage of each phase cells (right) in the indicated cells assessed by FCM. F and G. Western blot analyses of β-catenin, cyclin D1, SND1 and p21WAF1 (left), and comparisons among groups of their expressions (right) in the cells as indicated. Their relative expression levels were normalized against β-actin. All the experiments were performed at least in triplicate and the data are presented as the mean ± SD. * P<0.05, **P<0.01, ***/▲▲▲ P<0.001. Compared with Scr group* and with miR-320a group▲ in FCM data.