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. 2017 Feb 28;8(12):19834–19842. doi: 10.18632/oncotarget.15771

Figure 1. Apigenin inhibits ACHN, 786-0, and Caki-1 cell proliferation.

Figure 1

CCK-8 analysis showed apigenin inhibited proliferation in ACHN, 786-0, and Caki-1 RCC cells, and the normal renal proximal tubule epithelial cell line, HK-2, in a dose- and time-dependent manner (A) This analysis was repeated three times, with six replicates per experiment. Representative colony formation assay images and quantitation (B). Cell counts > 50 were defined as a colony. Representative EdU proliferation assay images using a 10× objective (C) ACHN cell proliferation rates were calculated as the ratios of EdU-staining cells to Hoechst33342-staining cells from six fields of three separate experiments. Scale bar = 25 μm. *P < 0.05, **P < 0.01 versus untreated control.