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. 2017 Feb 28;8(12):19834–19842. doi: 10.18632/oncotarget.15771

Figure 2. Apigenin treatment induces DNA damage in ACHN cells.

Figure 2

Comet assay using 4S Green Plus dye following cell treatment with various apigenin concentrations (A) Red arrows specify cells with a “comet tail,” which indicates DNA damage. Scale bar = 25 μm. Comet formation incidences in each group were measured using 100 randomly selected cells, and DNA damage level was calculated using CaspLab software in accordance with % tail DNA (B–C). Western blotting analysis of γH2AX D. *P < 0.05, **P < 0.01 versus untreated control.