Figure 5. Apigenin inhibits tumorigenicity in ACHN cell xenograft nude mice.

Tumor xenograft model (A) ACHN cells (1 × 10⁶ in 100 μl PBS) were injected subcutaneously into the right flank of each mouse. After palpable tumors arose, apigenin (or vehicle alone) was administered at 30 mg/kg every 3 d intraperitoneally. Tumors grew more slowly in apigenin-treated mice. Harvested tumors represented smaller ones in apigenin-treated group (B). Apigenin treatment did not affect end point RBC and WBC counts (C). Apigenin treated tumors had reduced Ki-67 expression (D). Scale bar = 100 μm. Error bars represent the S.D. from five nude mice. *P < 0.05, **P < 0.01.