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. 2017 Feb 8;8(12):19866–19878. doi: 10.18632/oncotarget.15189

Figure 1. Important role of miR-214 in VSMCs differentiation from ESCs.

Figure 1

A. Up-regulated miR-214 during VSMCs differentiation from ESCs. Mouse ESCs were seeded on Collagen IV coated dishes and cultured in differentiated media, the expression of miR-214 during VSMCs differentiation was assessed through RT-qPCR analyses. (Data are means ± SEM. (n=3), *p<0.05. B-F. SMC-specific gene expression was modulated by miR-214. ESCs were induced to differentiate towards VSMCs for 4 days, and the differentiating ESCs were transfected with either miRNA mimics negative control (mimic control in short), miR-214 mimics, or miRNA inhibitor negative control (inhibitor control in short) or miR-214 inhibitor, respectively. Transfected cells were cultured in VSMCs differentiation medium for another 48 hours. Total RNAs or proteins were harvested and subjected to RT-qPCR (B-D) or Western Blotting (E-F) analyses, respectively. The data presented here are representative (E) or mean±S.E.M. of three to six independent experiments. *P<0.05.