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. 2017 Feb 8;8(12):19866–19878. doi: 10.18632/oncotarget.15189

Figure 3. Target gene, QKI, is negatively regulated by miR-214.

Figure 3

A-D. Modulations of miR-214 expression levels negatively regulate QKI gene expression. Day 2~3 differentiating ESCs were transfected with miR-214 mimic or inhibitor, or respective negative controls, and cultured in VSMCs differentiation medium for 48 to 72 hours. Total RNA and protein were harvested and subjected to RT-qPCR (A and C) and Western blot (B and D) analyses, respectively. (Data are means ± SEM. (n=3), *p<0.05). E-F. ESCs were forced to differentiate towards VSMCs for 4 days, and co-transfections of miR-214 mimic with the luciferase plasmid of the QKI-UTR-wt or QKI-UTR-mt were performed. Forty-eight hours later, cells were harvested and subjected to luciferase analyses, (data are means ± SEM. (n=3), *p<0.05, # p<0.01).