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. 2017 Feb 11;8(12):19968–19979. doi: 10.18632/oncotarget.15274

Figure 4. eIF3i selectively promotes VEGFR2 and ERK translation.

Figure 4

A. eIF3i siRNAs inhibited ERK/VEGFR2 signaling pathway in HUVECs. GAPDH was used as a loading control. Fold changes were compared with control-siRNA and presented below each blot. B. Polysomes from control siRNA- and eIF3i siRNA-transfected HUVECs were fractionated with sucrose gradients. Upper panel showed the schematic diagram of the 10-50% sucrose gradients, and lower panel showed the electrophoresis results of RNAs extracted from the fractions. C. Levels of PCR products of indicated genes were analyzed with agarose gel electrophoresis. D. VEGFR2, ERK, β-actin and GAPDH PCR products were quantified with ImageJ software. E. Overexpression of eIF3i increased the ERK and VEGFR2 protein level in HUVECs. Tubulin was used as a loading control. Fold changes were compared with Lvx and presented below each blot.