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. 2017 Feb 13;8(12):19980–19996. doi: 10.18632/oncotarget.15301

Figure 4. SNORA74B silencing promoted GBC cell apoptosis.

Figure 4

A. FACS determined the relative number of apoptotic cells after double staining with Annexin V and PI. X axis represents the level of Annexin-V FITC fluorescence and the Y axis the PI fluorescence. B. The percent of apoptotic cells for GBC-SD, SGC996, and NOZ (p<0.05). C. The number of TUNEL-positive cells for GBC-SD xenografts. D. Effects of SNORA74B silencing on apoptosis in vivo were detected using TUNEL assay. The blue fluorescence indicates nuclear staining (DAPI) and the red fluorescence represents TUNEL-positive nuclei of GBC-SD cells. E. Apoptosis regulators were examined using western blotting, and the values of relative density of the bands normalized to GAPDH and COX IV are shown.