Table 5. Relative gray value of protein bands after treatments in Figure 3E, 4E, 5A, 5B and 5C.
| Figure 3E | |||||
|---|---|---|---|---|---|
| Protein | Mock | sh-NC | sh-SNORA74B | sh-SNORA74B+sh-PHLPP | |
| cyclin D1 | 1 | 1.16 | 0.35 | 1.04 | |
| 1 | 1.06 | 0.46 | 0.96 | ||
| 1 | 1.02 | 0.42 | 0.94 | ||
| 1 | 1.08 | 0.41 | 0.98 | mean | |
| 0 | 0.072 | 0.056 | 0.053 | SD | |
| p21 | 1 | 1.15 | 2.06 | 1.09 | |
| 1 | 0.94 | 1.95 | 1.02 | ||
| 1 | 1.06 | 1.93 | 1.07 | ||
| 1 | 1.05 | 1.98 | 1.06 | mean | |
| 0 | 0.105 | 0.07 | 0.036 | SD | |
| p27 | 1 | 1.15 | 2.2 | 1.01 | |
| 1 | 0.99 | 2.11 | 0.93 | ||
| 1 | 1.22 | 2.14 | 0.94 | ||
| 1 | 1.12 | 2.15 | 0.96 | mean | |
| 0 | 0.118 | 0.046 | 0.044 | SD | |
| GAPDH | 1 | 1 | 1 | 1 | |
| Figure 4E | |||||
| bcl-2 | 1 | 0.97 | 0.35 | 0.91 | |
| 1 | 0.92 | 0.36 | 0.91 | ||
| 1 | 1.02 | 0.4 | 0.94 | ||
| 1 | 0.97 | 0.37 | 0.92 | mean | |
| 0 | 0.05 | 0.026 | 0.017 | SD | |
| bax | 1 | 0.85 | 1.86 | 0.96 | |
| 1 | 0.94 | 1.88 | 1.02 | ||
| 1 | 1.06 | 1.93 | 0.99 | ||
| 1 | 0.95 | 1.89 | 0.99 | mean | |
| 0 | 0.105 | 0.036 | 0.03 | SD | |
| procapase-3 | 1 | 0.93 | 0.98 | 1.05 | |
| 1 | 0.99 | 0.99 | 1.01 | ||
| 1 | 0.93 | 0.79 | 0.94 | ||
| 1 | 0.95 | 0.92 | 1 | mean | |
| 0 | 0.034641 | 0.1126943 | 0.0556776 | SD | |
| cleaved caspase-3 | 1 | 0.89 | 1.98 | 0.91 | |
| 1 | 1.05 | 2.11 | 1.03 | ||
| 1 | 0.88 | 2 | 0.73 | ||
| 1 | 0.94 | 2.03 | 0.89 | mean | |
| 0 | 0.095 | 0.07 | 0.151 | SD | |
| GAPDH | 1 | 1 | 1 | 1 | |
| mitochondrial cytochrome C | 1 | 0.99 | 0.41 | 1.05 | |
| 1 | 0.99 | 0.42 | 1.09 | ||
| 1 | 1.26 | 0.46 | 1.16 | ||
| 1 | 1.08 | 0.43 | 1.1 | mean | |
| 0 | 0.156 | 0.026 | 0.056 | SD | |
| cytosolic cytochrome C | 1 | 0.99 | 2.08 | 1.05 | |
| 1 | 1.03 | 2.15 | 1.03 | ||
| 1 | 1.16 | 2.19 | 1.04 | ||
| 1 | 1.06 | 2.14 | 1.04 | mean | |
| 0 | 0.089 | 0.056 | 0.01 | SD | |
| COXIV | 1 | 1 | 1 | 1 | |
| Figure 5A | |||||
| AKT | 1 | 1.03 | 1.09 | 1.06 | |
| 1 | 1.07 | 1.08 | 1.12 | ||
| 1 | 1.02 | 0.98 | 1.06 | ||
| 1 | 1.04 | 1.05 | 1.08 | mean | |
| 0 | 0.026 | 0.061 | 0.035 | SD | |
| p-AKT | 1 | 0.8 | 0.29 | 0.96 | |
| 1 | 1.05 | 0.4 | 1.02 | ||
| 1 | 1.06 | 0.36 | 1.08 | ||
| 1 | 0.97 | 0.35 | 1.02 | mean | |
| 0 | 0.1473092 | 0.0556776 | 0.06 | SD | |
| Mtor | 1 | 0.93 | 0.98 | 1.05 | |
| 1 | 0.99 | 0.99 | 1.01 | ||
| 1 | 1.14 | 1.06 | 0.85 | ||
| 1 | 1.02 | 1.01 | 0.97 | mean | |
| 0 | 0.108 | 0.044 | 0.106 | SD | |
| p-Mtor | 1 | 0.99 | 0.19 | 0.98 | |
| 1 | 1.05 | 0.28 | 1.03 | ||
| 1 | 1.2 | 0.34 | 1.14 | ||
| 1 | 1.09 | 0.27 | 1.05 | mean | |
| 0 | 0.108 | 0.0753 | 0.082 | SD | |
| 4EBP1 | 1 | 1.03 | 1.01 | 0.99 | |
| 1 | 1.05 | 1.09 | 1.03 | ||
| 1 | 1.13 | 1.14 | 1.04 | ||
| 1 | 1.07 | 1.08 | 1.02 | mean | |
| 0 | 0.053 | 0.066 | 0.026 | SD | |
| p-4EBP1 | 1 | 0.98 | 0.34 | 0.89 | |
| 1 | 1 | 0.35 | 1.02 | ||
| 1 | 1.05 | 0.39 | 1.03 | ||
| 1 | 1.01 | 0.36 | 0.98 | mean | |
| 0 | 0.036 | 0.026 | 0.078 | SD | |
| p70 S6K | 1 | 0.93 | 0.91 | 0.98 | |
| 1 | 0.95 | 0.94 | 1.01 | ||
| 1 | 1.12 | 1.03 | 1.13 | ||
| 1 | 1 | 0.96 | 1.04 | mean | |
| 0 | 0.104 | 0.062 | 0.079 | SD | |
| p-p70 S6K | 1 | 0.96 | 0.42 | 1.01 | |
| 1 | 0.95 | 0.41 | 1.03 | ||
| 1 | 1.15 | 0.52 | 1.11 | ||
| 1 | 1.02 | 0.45 | 1.05 | mean | |
| 0 | 0.113 | 0.061 | 0.053 | SD | |
| bad | 1 | 0.86 | 0.98 | 1.02 | |
| 1 | 0.85 | 1.01 | 0.93 | ||
| 1 | 1.05 | 1.07 | 0.87 | ||
| 1 | 0.92 | 1.02 | 0.95 | mean | |
| 0.113 | 0.046 | 0.075 | SD | ||
| p-bad | 1 | 0.9 | 0.45 | 0.96 | |
| 1 | 0.96 | 0.47 | 1.03 | ||
| 1 | 0.96 | 0.52 | 1.04 | ||
| 1 | 0.94 | 0.48 | 1.01 | mean | |
| 0 | 0.035 | 0.036 | 0.044 | SD | |
| GAPDH | 1 | 1 | 1 | 1 | |
| Figure 5B | |||||
| PTEN | 1 | 0.95 | 0.92 | 0.91 | |
| 1 | 0.96 | 0.94 | 0.92 | ||
| 1 | 1 | 1.02 | 0.96 | ||
| 1 | 0.97 | 0.96 | 0.93 | mean | |
| 0 | 0.026 | 0.053 | 0.026 | SD | |
| CTMP | 1 | 0.97 | 1.06 | 1.01 | |
| 1 | 0.97 | 1.05 | 1.03 | ||
| 1 | 1.09 | 1.13 | 1.14 | ||
| 1 | 1.01 | 1.08 | 1.06 | mean | |
| 0 | 0.069 | 0.044 | 0.07 | SD | |
| PHLPP | 1 | 1.01 | 2.51 | 0.96 | |
| 1 | 1.05 | 2.68 | 0.99 | ||
| 1 | 1.09 | 2.7 | 0.99 | ||
| 1 | 1.05 | 2.63 | 0.98 | mean | |
| 0 | 0.04 | 0.104 | 0.017 | SD | |
| GAPDH | 1 | 1 | 1 | 1 | |
| Figure 5C | |||||
| PHLPP | 1 | 0.2 | 0.09 | ||
| 1 | 0.24 | 0.1 | |||
| 1 | 0.25 | 0.14 | |||
| 1 | 0.23 | 0.11 | mean | ||
| 0 | 0.026 | 0.0265 | SD | ||
| PHLPP | 1 | 0.18 | 0.06 | ||
| 1 | 0.19 | 0.09 | |||
| 1 | 0.2 | 0.09 | |||
| 1 | 0.19 | 0.08 | mean | ||
| 0 | 0.01 | 0.017 | SD | ||
| PHLPP | 1 | 0.15 | 0.08 | ||
| 1 | 0.18 | 0.08 | |||
| 1 | 0.18 | 1.11 | |||
| 1 | 0.17 | 0.09 | mean | ||
| 0 | 0.017 | 0.595 | SD | ||
| GAPDH | 1 | 1 | 1 | ||