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. 2017 Feb 16;8(12):20067–20085. doi: 10.18632/oncotarget.15379

Figure 7. A-SMase interacts with caspases-3 or caspases-7.

Figure 7

a. Immunoprecipitation of A-SMase with an anti-A-SMase antibody from unstimulated and SFF-CD95L stimulated HeLa wild-type cell lysates. Western blots show that pro-caspase-3, pro-caspase-7 and cleaved-caspase-7 co-precipitates with A-SMase. b. Immunofluorescence analysis visualize a co-localization (yellow) between A-SMase (red) and caspases-3 or caspases-7 (green) in unstimulated SKW6.4 cells. c. Activation of immunoprecipitated A-SMase-GFP after 15 min incubation with caspase-3 or caspase-7 (n=3, 1way Anova with Bonferroni multiple comparison test).