Figure 5. Effect of cabozantinib on osteoclast formation on cabozantinib pre-treated osteoblast layer.

TRAP-positive cells (red) identify osteoclasts. Representative images: A. Osteoblasts cultured without osteoclasts; B. Osteoblasts co-cultured with osteoclasts; C. Osteoblasts pre-treated with cabozantinib (3 μM) and co-cultured with osteoclasts; D. Osteoblasts pre-treated with cabozantinib (5 μM) and co-cultured with osteoclasts; E. Number of TRAP-positive cells counted in the three different conditions (B,C and D). F and G. Higher magnification of pictures B and D respectively