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. 2017 Feb 17;8(12):20133–20144. doi: 10.18632/oncotarget.15448

Figure 5. miR-363-3p inhibits the expression of PCNA by binding its 3′UTR.

Figure 5

A. The protein levels of PCNA was examined by Western blotting for three cell lines infected with either lenti-NC, lenti-363-mimic or lenti-363-inhibitor and quantification of the results in panel A. B. Expression of PCNA in lung cancer tissues compared with adjacent non-cancerous tissues. GAPDH was used as an internal control. (** P < 0.01 vs. Normal). C. The predicted miR-363-3p binding site in the 3′UTR of the PCNA mRNA was cloned into psiCHECK2 reporter vector, and a corresponding mutant was constructed. The constructs were then transfected into 293T cells, which were infected with either lenti-NC or lenti-363-mimics. The data are expressed as the means ± SEM (n = 3), with results representative of 3 independent experiments. (* p < 0.05 and ** p < 0.01 vs. NC).