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. 2017 Jan 27;8(12):20266–20287. doi: 10.18632/oncotarget.14865

Table 3. Assignment of specific Raman spectral components to subcellular constituents.

Raman Shift (cm−1) DNA/RNA Proteins Lipids
620 C-C Twist Aromatic Ring
643 C-C Twisting of Tyrosine
702 Cholesterol
715 Adenine C-N (Membrane Phospholipid Head Group)/C-N-(CH3)3
781 Cytosine/Uracil Ring Breathing
810 Phosphodiester
828 O-P-O Stretching Tyrosine Phosphodiester
853 Ring Breathing of Tyrosine C-C Stretch of Proline Ring
878 C-C-N+ Symmetric Stretching
938 Hydroxyproline/Proline ν(C-C) Vibration of Collagen Backbone
1005 Phenylalanine
1035 Collagen
1066 Proline Fatty Acid
1086 ν1CO32−, ν3PO43−, ν(C-C)Acyl Backbone
1128 C-N Stretching
1156 C-C, C-N Stretching
1176 C-H Bending Tyrosine
1209 Tryptophan & Phenylalanine ν(C-C6H5)
1241 Asymmetric Phosphate Stretching
1254 C-N in Plane Stretching
1266 Amide III (α-Helix)/Tryptophan/Collagen
1302 CH3/δ(CH2) Twisting, Wagging, Collagen, Amide III, & -CH2- Bending CH3/δ(CH2) Twisting, Wagging, Phospholipids, &-CH2- Bending
1334 Nucleic Acid CH3CH2 Wagging, Collagen
1342 CH Deformation
1367 vs (CH3) Phospholipids
1391 C-N Stretching in Quinoid Ring-Benzoid
1437 CH2 Deformation
1451 CH2 Bending/CH3 Bending C-H Deformations C-H Deformation
1556 Tryptophan ν(CN) & Amide II ν(C=C) Porphyrin & Tyrosine
1605 Cytosine Phenylalanine & Tyrosine
1657 ν(C=O) Amide I (α-Helix) C=O Stretching of Collagen & Elastin C=C Stretch Fatty Acids