Figure 13. MCF7 cells harboring GCLC show a significant increase in mitochondrial oxygen consumption and mitochondrial ATP production.
The Seahorse XF96 analyzer was employed to determine the mitochondrial function of MCF-7-LV105 (empty vector) and MCF-7-GCLC cells after 48 hours. A. A representative line graph of 3 independent experiments is shown. B. Respiration (basal and maximal), as well as ATP levels, were significantly increased.** p < 0.005; *** p < 0.0005.