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Levofloxacin treatment leads to increased Caspase-3 activity which is reduced by pretreatment with sulforaphane. Caspase-3 activity was detected by colorimetric assay after SNECs were treated for 48-hours levofloxacin (0,20,40, or 80μg/mL) alone (black squares), or pretreated for 24 hours with 10μM sulforaphane (open circles). Colorimetric output of the assay is shown as arbitrary units (A.U.) +/− the standard error of the mean (s.e.m). * indicates p< 0.05 +/− sulforaphane treatment.
