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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: J Autoimmun. 2017 Jan 23;79:39–52. doi: 10.1016/j.jaut.2017.01.003

Fig. 1.

Fig. 1

Topical MC903 treatment increases Treg numbers independent of VDR expression on hematopoietic cells. (A) C57BL/6 mice were treated with ethanol (EtOH) or MC903 (2nmol/ear) once daily for 7 days. On day 8, mice were euthanized and analyzed. (B) Representative flow plots of live CD4+ cells from spleens of EtOH and MC903 treated mice at day 8. (C) The percent of Tregs of CD4+ T cells in blood, spleen, skin-draining lymph nodes (dLN) and skin at day 8 is plotted as mean ± SEM (n = 6–10 mice/group from 4 independent experiments). (D) Total Treg numbers in the spleen and dLN at day 8 are plotted as mean ± SEM (n = 6–10 mice/group from 3 independent experiments). (E) The percent of Tregs of CD4+ T cells at day 8 from mice treated with EtOH or MC903 topically or i.p. is plotted as mean ± SEM (n = 5–6 mice/group from 2 independent experiments). (F) WT and VDR KO BM chimeras were treated with EtOH or MC903. ) The percent of Tregs of CD4+ T cells in spleen on day 8 after treatment is plotted as mean ± SEM (n = 4–5/group from 2 independent experiments) *p<0.05 and ***p<0.001 by Student’s t test.