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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Ocul Surf. 2016 Dec 29;15(2):193–201. doi: 10.1016/j.jtos.2016.12.002

Figure 3.

Figure 3

Non-invasive microvascular perfusion maps (nMPMs) and fractal analysis. Custom software was developed to segment conjunctival vessels and to create the nMPMs for fractal analysis using a series of image processing procedures. The raw image was first resized from 3,456 × 2,304 pixels to 1,024 × 683 pixels (A). Imaging processing using morphological opening was performed to create nMPMs (B). Vessels were segmented (C) and the image was cropped a field of view 7.85 × 7.85 mm2 containing 512 × 512 pixels (D). The cropped image was then inverted (E) and skeletonized (F). The image was further inverted back for fractal analysis (G) using monofractal analysis (i.e. box-counting, H). Bars=3 mm.