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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: World J Biol Psychiatry. 2016 Oct 10;18(6):445–456. doi: 10.1080/15622975.2016.1224927

Figure 4.

Figure 4

Inhibition of GSK3 attenuates depression-like behaviours and up-regulates expression of the 5HTR2C miRNA cluster. (a) Escape failures in the learned helplessness test for wild-type mice treated with intranasal administration of vehicle (n = 21) or L803-mts (n = 12). Each symbol represents results from an individual mouse. The dashed line at 15 escape failures indicates the criteria for learned helplessness (Student’s t-test; t(31) = 2.958; *P <.05). (b) Latency to feed in the NSF test with (closed bars) or without (open bars) induction of learned helplessness (LH) in wild-type mice treated intranasally with vehicle (Veh) or L803-mts. Data represent means ± SEM, n = 8–10 (two-way ANOVA (LH X treatment); Finteraction(1,32) = 4.181; Bonferroni post-hoc test, *P <.05, compared to the vehicle-treated control mice, **P <.05, compared to vehicle-treated learned helplessness mice). (c) Expression levels of the 5HTR2C cluster miRNAs (764-5p, 1912-3p, 1264-3p, 1298-5p and 448-3p) in the hippocampus from wild-type mice treated intranasally with vehicle (black bars) or L803-mts (white bars). Data represent means ± SEM, n = 4 (Student’s t-test; 1912-3p: t(6) = 2.547; 1264-3p: t(6) = 2.589; 1298-5p: t(6) = 2.578; 448-3p: t(6) = 2.608; *<0.05).